Development of BIOnanotechnologies based on Extracellular Vesicles for early diagnosis, prognosis and therapy of atherosclerotic disease (BIOVEA)
Various biomarkers have been proposed for the diagnosis and prognosis of acute coronary syndrome, however none for stable coronary disease. Identification of biomarkers that augment established clinical indicators, may allow earlier diagnosis of coronary disease, as well as improved recognition of the risk of complications and cardiovascular death. Additionally, this would pave the way for biomarkers, such as extracellular vesicles, to be employed as precise therapeutic agents in these diseases.
SELECTED PUBLICATIONS (MOST RECENT FIRST):
2023
Obrișcă B, Mocanu V, Vornicu A, Jurubiță R, Sorohan B, Dimofte G, Achim C, Andronesi A, Micu G, Bobeică R, Caceaune N, Procop A, Herlea V, Gherghiceanu M, Ismail G. The impact of SARS-CoV-2 infection on renal function in patients with biopsy-proven kidney diseases. PLOS ONE. DOI: 10.1371/journal.pone.0296168.
Balbi C, Parisse P, Vondracek H, Lazzarini E, Bolis S, Fertig TE, Gherghiceanu M, Barile L, Vassalli G. Impact of Isolation Methods on Extracellular Vesicle Functionality In Vitro and In Vivo. Advanced Biology 8(2). DOI: 10.1002/adbi.202300185.
Pecquet C, Papadopoulos N, Balligand T, Chachoua I, Tisserand A, Vertenoeil G, Nédélec A, Vertommen D, Roy A, Marty C, Nivarthi H, Defour J-P, El-Khoury M, Hug E, Majoros A, Xu E, Zagrijtschuk O, Fertig TE, Marta DS, Gisslinger H, Gisslinger B, Schalling M, Casetti IC, Rumi E, Pietra D, Cavalloni C, Arcaini L, Cazzola M, Komatsu N, Kihara Y, Sunami Y, Edahiro Y, Araki M, Lesyk R, Buxhofer-Ausch V, Heibl S, Pasquier F, Plo I, Vainchenker W, Kralovics R, Constantinescu SN. Secreted Mutant Calreticulins As Rogue Cytokines in Myeloproliferative Neoplasms. Blood 141(8):917-929. DOI: 10.1182/blood.2022016846.
2022
Fertig TE, Chitoiu L, Marta DS, Ionescu VS, Cismasiu VB, Radu E, Angheluta G, Dobre M, Serbanescu A, Hinescu ME, Gherghiceanu M. Vaccine mRNA Can Be Detected in Blood at 15 Days Post-Vaccination. Biomedicines. DOI: 10.3390/biomedicines10071538.
2021
Balbi C, Milano G, Fertig TE, Lazzarini E, Bolis S, Taniyama Y, Sanada F, Di Silvestre D, Mauri P, Gherghiceanu M, Lüscher TF, Barile L, Vassalli G. An exosomal-carried short periostin isoform induces cardiomyocyte proliferation. Theranostics. DOI:10.7150/thno.57243.
2020
Sorop A, Iacob R, Iacob S, Constantinescu D, Chitoiu L, Fertig TE, Dinischiotu A, Chivu-Economescu M, Bacalbasa N, Savu L, Gheorghe L, Dima S, Popescu I. Plasma Small Extracellular Vesicles Derived miR-21-5p and miR-92a-3p as Potential Biomarkers for Hepatocellular Carcinoma Screening. Frontiers in Genetics. DOI:10.3389/fgene.2020.00712.
2019
Ciullo A, Biemmi V, Milano G, Bolis S, Cervio E, Fertig ET, Gherghiceanu M, Moccetti T, Camici GG, Vassalli G, Barile L. Exosomal Expression of CXCR4 Targets Cardioprotective Vesicles to Myocardial Infarction and Improves Outcome after Systemic Administration. International Journal of Molecular Sciences. DOI: 10.3390/ijms20030468
Ceafalan LC, Fertig TE, Gheorghe TC, Hinescu ME, Popescu BO, Pahnke J, Gherghiceanu M. Age-related ultrastructural changes of the basement membrane in the mouse blood-brain barrier. Journal of Cellular and Molecular Medicine. DOI: 10.1111/jcmm.13980
2018
Pecquet C, Balligand T, Chachoua I, Roy A, Vertenoeil G, Colau D, Fertig E, Marty C, Nivarthi H, Defour J-P, Xu E, Hug E, Gisslinger H, Gisslinger B, Schalling M, Casetti IC, Rumi E, Pietra D, Cavalloni C, Arcaini L, Cazzola M, Komatsu N, Kihara Y, Sunami Y, Edahiro Y, Araki M, Plo I, Vainchenker W, Kralovics R, Constantinescu SN. Secreted Mutant Calreticulins As Rogue Cytokines Trigger Thrombopoietin Receptor Activation Specifically in CALR Mutated Cells: Perspectives for MPN Therapy. Blood. DOI: 10.1182/blood-2018-99-118348
Barile L, Cervio E, Lionetti V, Milano G, Ciullo A, Biemmi V, Bolis S, Altomare C, Matteucci M, Di Silvestre D, Brambilla F, Fertig TE, Torre T, Demertzis S, Mauri P, Moccetti T, Vassalli G. Cardioprotection by cardiac progenitor cell-secreted exosomes: role of pregnancy-associated plasma protein-A. Cardiovascular Research. DOI: 10.1093/cvr/cvy055.
2017
Ceafalan LC, Fertig TE, Popescu AC, Popescu BO, Hinescu ME, Gherghiceanu M. Skeletal muscle regeneration involves macrophage-myoblast bonding. Cell Adhesion & Migration. DOI: 10.1080/19336918.2017.1346774
2015
Popescu LM, Curici A, Wang E, Zhang H, Hu S, Gherghiceanu M. Telocytes and putative stem cells in ageing human heart. Journal of Cellular and Molecular Medicine. DOI: 10.1111/jcmm.12509
2014
Fertig ET, Gherghiceanu M, Popescu LM. Extracellular vesicles release by cardiac telocytes: electron microscopy and electron tomography. Journal of Cellular and Molecular Medicine. DOI: 10.1111/jcmm.12436
Galiger C, Kostin S, Golec A, Ahlbrecht K, Becker S, Gherghiceanu M, Popescu LM, Morty RE, Seeger W, Voswinckel R. Phenotypical and ultrastructural features of Oct4-positive cells in the adult mouse lung. Journal of Cellular and Molecular Medicine. DOI: 10.1111/jcmm.12295
2012
Barile L, Gherghiceanu M, Popescu LM, Moccetti T, Vassalli G. Ultrastructural Evidence of Exosome Secretion by Progenitor Cells in Adult Mouse Myocardium and Adult Human Cardiospheres. Journal of Biomedicine and Biotechnology. DOI: 10.1155/2012/354605
Gherghiceanu M & Popescu LM. Cardiac telocytes - their junctions and functional implications. Cell and Tissue Research. DOI: 10.1007/s00441-012-1333-8.
2011
Gherghiceanu M, Barad L, Novak A, Reiter I, Itskovitz-Eldor J, Binah O, Popescu LM. Cardiomyocytes derived from human embryonic and induced pluripotent stem cells: comparative ultrastructure. Journal of Cellular and Molecular Medicine. DOI: 10.1111/j.1582-4934.2011.01417.x
Popescu LM, Gherghiceanu M, Suciu LC, Manole CG, Hinescu ME. Telocytes and putative stem cells in the lungs: electron microscopy, electron tomography and laser scanning microscopy. Cell and Tissue Research. DOI: 10.1007/s00441-011-1229-z
Gherghiceanu M & Popescu LM. Heterocellular communication in the heart: electron tomography of telocyte-myocyte junctions. Journal of Cellular and Molecular Medicine. DOI: 10.1111/j.1582-4934.2011.01299.x
Hinescu ME, Gherghiceanu M, Suciu L, Popescu LM. Telocytes in pleura: two- and three-dimensional imaging by transmission electron microscopy. Cell and Tissue Research. DOI: 10.1007/s00441-010-1095-0
2010
Mandache E, Gherghiceanu M, Macarie C, Kostin S, Popescu LM. Telocytes in human isolated atrial amyloidosis: ultrastructural remodelling. Journal of Cellular and Molecular Medicine. DOI: 10.1111/j.1582-4934.2010.01200.x
Bani D, Formigli L, Gherghiceanu M, Faussone-Pellegrini MS. Telocytes as supporting cells for myocardial tissue organization in developing and adult heart. Journal of Cellular and Molecular Medicine. DOI: 10.1111/j.1582-4934.2010.01119.x
Gherghiceanu M, Manole CG, Popescu LM. TELOCYTES IN ENDOCARDIUM: Electron Microscope Evidence. Journal of Cellular and Molecular Medicine. DOI: 10.1111/j.1582-4934.2010.01133.x
Gherghiceanu M & Popescu LM. Cardiomyocyte precursors and telocytes in epicardial stem cell niche: electron microscope images. Journal of Cellular and Molecular Medicine. DOI: 10.1111/j.1582-4934.2010.01060.x
2009
Popescu LM, Gherghiceanu M, Manole CG, Faussone-Pellegrini MS. Cardiac renewing: interstitial Cajal-like cells nurse cardiomyocyte progenitors in epicardial stem cell niches. Journal of Cellular and Molecular Medicine. DOI: 10.1111/j.1582-4934.2009.00758.x
Gurusamy N, Lekli I, Gorbunov NV, Gherghiceanu M, Popescu LM, Das DK. Cardioprotection by adaptation to ischaemia augments autophagy in association with BAG-1 protein. Journal of Cellular and Molecular Medicine. DOI: 10.1111/j.1582-4934.2008.00495.x
2007
Gherghiceanu M & Popescu LM. Electron microscope tomography: further demonstration of nanocontacts between caveolae and smooth muscle sarcoplasmic reticulum. Journal of Cellular and Molecular Medicine. DOI: 10.1111/j.1582-4934.2007.00166.x
Mandache E, Popescu LM, Gherghiceanu M. Myocardial interstitial Cajal-like cells (ICLC) and their nanostructural relationships with intercalated discs: shed vesicles as intermediates. Journal of Cellular and Molecular Medicine. DOI: 10.1111/j.1582-4934.2007.00117.x
Hinescu ME, Ardeleanu C, Gherghiceanu M, Popescu LM. Interstitial Cajal-like cells in human gallbladder. Journal of Molecular Histology. DOI: 10.1007/s10735-007-9099-0
2006
Popescu LM, Gherghiceanu M, Mandache E, Cretoiu D. Caveolae in smooth muscles: nanocontacts. Journal of Cellular and Molecular Medicine. DOI: 10.1111/j.1582-4934.2006.tb00539.x
Popescu LM, Gherghiceanu M, Hinescu ME, Cretoiu D, Ceafalan L, Regalia T, Popescu AC, Ardeleanu C, Mandache E. Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC). Journal of Cellular and Molecular Medicine. DOI: 10.1111/j.1582-4934.2006.tb00410.x
Hinescu ME, Gherghiceanu M, Mandache E, Ciontea SM, Popescu LM. Interstitial Cajal-like cells (ICLC) in atrial myocardium: ultrastructural and immunohistochemical characterization. Journal of Cellular and Molecular Medicine. DOI: 10.1111/j.1582-4934.2006.tb00306.x
2005
Popescu LM, Gherghiceanu M, Cretoiu D, Radu E. The connective connection: interstitial cells of Cajal (ICC) and ICC-like cells establish synapses with immunoreactive cells. Electron microscope study in situ. Journal of Cellular and Molecular Medicine. DOI: 10.1111/j.1582-4934.2005.tb00502.x
2004
Mandache E & Gherghiceanu M. Ultrastructural defects of the glomerular basement membranes associated with primary glomerular nephropathies. ULTRASTRUCTURAL PATHOLOGY. DOI: 10.1080/01913120490445016