Development of BIOnanotechnologies based on Extracellular Vesicles for early diagnosis, prognosis and therapy of atherosclerotic disease (BIOVEA)

Various biomarkers have been proposed for the diagnosis and prognosis of acute coronary syndrome, however none for stable coronary disease. Identification of biomarkers that augment established clinical indicators, may allow earlier diagnosis of coronary disease, as well as improved recognition of the risk of complications and cardiovascular death. Additionally, this would pave the way for biomarkers, such as extracellular vesicles, to be employed as precise therapeutic agents in these diseases.

ULTRASTRUCTURAL PATHOLOGY AND BIOIMAGING LAB

SELECTED PUBLICATIONS (MOST RECENT FIRST):

Ceafalan LC, Fertig TE, Gheorghe TC, Hinescu ME, Popescu BO, Pahnke J, Gherghiceanu M. Age-related ultrastructural changes of the basement membrane in the mouse blood-brain barrier. Journal of Cellular and Molecular Medicine, 2018
DOI: 10.1111/jcmm.13980

Barile L, Cervio E, Lionetti V, Milano G, Ciullo A, Biemmi V, Bolis S, Altomare C, Matteucci M, Di Silvestre D, Brambilla F, Fertig TE, Torre T, Demertzis S, Mauri P, Moccetti T, Vassalli G. Cardioprotection by cardiac progenitor cell-secreted exosomes: role of pregnancy-associated plasma protein-A. Cardiovascular Research 114(7): 992-1005, 2018.
DOI: 10.1093/cvr/cvy055

Ceafalan LC, Fertig TE, Popescu AC, Popescu BO, Hinescu ME, Gherghiceanu M. Skeletal muscle regeneration involves macrophage-myoblast bonding. Cell Adhesion & Migration, 2017.

DOI: 10.1080/19336918.2017.1346774

Popescu LM, Curici A, Wang E, Zhang H, Hu S, Gherghiceanu M. Telocytes and putative stem cells in ageing human heart. Journal of Cellular and Molecular Medicine 19(1): 31-45, 2015.
DOI: 10.1111/jcmm.12509

Fertig ET, Gherghiceanu M, Popescu LM. Extracellular vesicles release by cardiac telocytes: electron microscopy and electron tomography. Journal of Cellular and Molecular Medicine 18(10): 1938-1943, 2014.

DOI: 10.1111/jcmm.12436

Barile L, Lionetti V, Cervio E, Matteucci M, Gherghiceanu M, Popescu LM, Torre T, Siclari F, Moccetti T, Vassalli G. Extracellular vesicles from human cardiac progenitor cells inhibit cardiomyocyte apoptosis and improve cardiac function after myocardial infarction. Cardiovascular Research 103(4): 530-541, 2014.
DOI: 10.1093/cvr/cvu167

Galiger C, Kostin S, Golec A, Ahlbrecht K, Becker S, Gherghiceanu M, Popescu LM, Morty RE, Seeger W, Voswinckel R. Phenotypical and ultrastructural features of Oct4-positive cells in the adult mouse lung. Journal of Cellular and Molecular Medicine 18(7): 1321-1333, 2014.

DOI: 10.1111/jcmm.12295

Barile L, Gherghiceanu M, Popescu LM, Moccetti T, Vassalli G. Ultrastructural Evidence of Exosome Secretion by Progenitor Cells in Adult Mouse Myocardium and Adult Human Cardiospheres. Journal of Biomedicine and Biotechnology. Article Number: 354605, 2012.
DOI: 10.1155/2012/354605

Gherghiceanu M & Popescu LM. Cardiac telocytes - their junctions and functional implications. Cell and Tissue Research 348(2): 265-279, 2012.

DOI: 10.1007/s00441-012-1333-8.

Gherghiceanu M, Barad L, Novak A, Reiter I, Itskovitz-Eldor J, Binah O, Popescu LM. Cardiomyocytes derived from human embryonic and induced pluripotent stem cells: comparative ultrastructure. Journal of Cellular and Molecular Medicine 15(11): 2539-2551, 2011.

DOI: 10.1111/j.1582-4934.2011.01417.x

Popescu LM, Gherghiceanu M, Suciu LC, Manole CG, Hinescu ME. Telocytes and putative stem cells in the lungs: electron microscopy, electron tomography and laser scanning microscopy. Cell and Tissue Research 345(3): 391-403, 2011.

DOI: 10.1007/s00441-011-1229-z.

Gherghiceanu M & Popescu LM. Heterocellular communication in the heart: electron tomography of telocyte-myocyte junctions. Journal of Cellular and Molecular Medicine 15(4): 1005-1011, 2011.

DOI: 10.1111/j.1582-4934.2011.01299.x

Hinescu ME, Gherghiceanu M, Suciu L, Popescu LM. Telocytes in pleura: two- and three-dimensional imaging by transmission electron microscopy. Cell and Tissue Research 343(2): 389-397, 2011.

DOI: 10.1007/s00441-010-1095-0.

Mandache E, Gherghiceanu M, Macarie C, Kostin S, Popescu LM. Telocytes in human isolated atrial amyloidosis: ultrastructural remodelling. Journal of Cellular and Molecular Medicine 14(12): 2739-2747, 2010.

DOI: 10.1111/j.1582-4934.2010.01200.x

Bani D, Formigli L, Gherghiceanu M, Faussone-Pellegrini MS. Telocytes as supporting cells for myocardial tissue organization in developing and adult heart. Journal of Cellular and Molecular Medicine 14(10): 2531-2538, 2010.

DOI: 10.1111/j.1582-4934.2010.01119.x

Gherghiceanu M, Manole CG, Popescu LM. TELOCYTES IN ENDOCARDIUM: Electron Microscope Evidence. Journal of Cellular and Molecular Medicine 14(9): 2330-2334, 2010.

DOI: 10.1111/j.1582-4934.2010.01133.x

Gherghiceanu M & Popescu LM. Cardiomyocyte precursors and telocytes in epicardial stem cell niche: electron microscope images. Journal of Cellular and Molecular Medicine 14(4): 871-877, 2010.

DOI: 10.1111/j.1582-4934.2010.01060.x

Popescu LM, Gherghiceanu M, Manole CG, Faussone-Pellegrini MS. Cardiac renewing: interstitial Cajal-like cells nurse cardiomyocyte progenitors in epicardial stem cell niches. Journal of Cellular and Molecular Medicine 13(5): 866-886, 2009.

DOI: 10.1111/j.1582-4934.2009.00758.x

Gurusamy N, Lekli I, Gorbunov NV, Gherghiceanu M, Popescu LM, Das DK. Cardioprotection by adaptation to ischaemia augments autophagy in association with BAG-1 protein. Journal of Cellular and Molecular Medicine 13(2): 373-387, 2009.

DOI: 10.1111/j.1582-4934.2008.00495.x

Gherghiceanu M & Popescu LM. Electron microscope tomography: further demonstration of nanocontacts between caveolae and smooth muscle sarcoplasmic reticulum. Journal of Cellular and Molecular Medicine 11(6): 1416-1418, 2007.

DOI: 10.1111/j.1582-4934.2007.00166.x

Mandache E, Popescu LM, Gherghiceanu M. Myocardial interstitial Cajal-like cells (ICLC) and their nanostructural relationships with intercalated discs: shed vesicles as intermediates. Journal of Cellular and Molecular Medicine 11(5): 1175-1184, 2007.

DOI: 10.1111/j.1582-4934.2007.00117.x

Hinescu ME, Ardeleanu C, Gherghiceanu M, Popescu LM. Interstitial Cajal-like cells in human gallbladder. Journal of Molecular Histology 38(4): 275-284, 2007.

DOI: 10.1007/s10735-007-9099-0

Popescu LM, Gherghiceanu M, Mandache E, Cretoiu D. Caveolae in smooth muscles: nanocontacts. Journal of Cellular and Molecular Medicine 10(4): 960-990, 2006.

DOI: 10.1111/j.1582-4934.2006.tb00539.x

Popescu LM, Gherghiceanu M, Hinescu ME, Cretoiu D, Ceafalan L, Regalia T, Popescu AC, Ardeleanu C, Mandache E. Insights into the interstitium of ventricular myocardium: interstitial Cajal-like cells (ICLC). Journal of Cellular and Molecular Medicine 10(2): 429-458, 2006.
DOI: 10.1111/j.1582-4934.2006.tb00410.x

Hinescu ME, Gherghiceanu M, Mandache E, Ciontea SM, Popescu LM. Interstitial Cajal-like cells (ICLC) in atrial myocardium: ultrastructural and immunohistochemical characterization. Journal of Cellular and Molecular Medicine 10(1): 243-257, 2006
DOI: 10.1111/j.1582-4934.2006.tb00306.x

Popescu LM, Gherghiceanu M, Cretoiu D, Radu E. The connective connection: interstitial cells of Cajal (ICC) and ICC-like cells establish synapses with immunoreactive cells. Electron microscope study in situ. Journal of Cellular and Molecular Medicine 9(3): 714-730, 2005.

DOI: 10.1111/j.1582-4934.2005.tb00502.x

Mandache E & Gherghiceanu M. Ultrastructural defects of the glomerular basement membranes associated with primary glomerular nephropathies. ULTRASTRUCTURAL PATHOLOGY 28(2): 103-108, 2004.

DOI: 10.1080/01913120490445016